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Curriculum vitae

Gloria Juan, PhD

1994: PhD in Pharmacy/Biochemistry, University of Valencia, Spain. Dissertation: "Cytometric analysis of metabolic injury and cell death in normal and transformed rat hepatocytes".

1992: MSc in Pharmacy, University of Valencia, Spain. Dissertation: "Application of flow cytometry to the metabolic study of isolated hepatocytes".

1989: BSc in Pharmacy, University of Valencia, Spain.

CAREER HISTORY

My scientific career has mostly focused on oncology research, first in basic research including graduate school at the University of Valencia (Spain), post-doctoral training and as assistant professor at New York Medical College (Valhalla, NY), and later, in translational and clinical research at Memorial Sloan-Kettering Cancer Center (NYC).  Since 2005 at Amgen, Inc. (Thousand Oaks, CA), I focused on applying my knowledge and expertise to the development of novel therapies, leading teams involved in the discovery, development and validation of pharmacodynamic and patient stratification biomarkers for preclinical and clinical studies with the purpose of enabling clinical ecisions. Also, my interest centered on engaging science and technical practices to address the study of immunotherapies for solid tumors.

April 2024 – Present, Scientific Communications Consultant

Vinaròs, Spain

  • Editorial support for life sciences researchers. 

May 2012 – May 2020, Principal Scientist

Amgen (Clinical Biomarkers and Diagnostics), Thousand Oaks, California

  • I served as the biomarker representative for cross-functional project teams in oncology, developing the translational biomarker strategy and execution; directly supervising assay design and application of relevant technologies; directing and managing the outsourcing and validation of related assays; and overseeing the implementation of biomarkers for patient selection and pharmacodynamics into clinical studies. 

May 2005 – May 2012, Senior Scientist (2005-2007) to Principal Scientist (2008-2012)

Amgen (Clinical Immunology), Thousand Oaks, California

  • I led teams involved in the discovery, development and validation of pharmacodynamic biomarkers.

  • Additionally, I was responsible of assessing the immunogenicity risk of biologics.

October 1999 – May 2005, Research Scientist 

Memorial Sloan-Kettering Cancer Center (MSKCC, Dept. Pathology), New York, NY

  • I applied state-of-the-art imaging techniques to cancer studies together with the analysis of cell cycle regulators in translational studies. 

August 1995 – May 1999, Postdoctoral Fellow (1995-1997) to Assistant Professor (1997-1999), New York Medical College (NYMC, Dept. Medicine), Valhalla, NY

  • I was involved in projects focused on the analysis of cell cycle regulation and apoptosis. My publications on immunocytochemical detection of pRB and histone H3 phosphorylation are the first in the literature.

FELLOWSHIPS AND AWARDS

  • Predoctoral fellowship, 1991-1993, University of Valencia.

  • Spanish Federation of Sport Medicine (FEMEDE), 1992

  • Scientific Foundation of the Spanish Association against Cancer, 1994

  • This Close fellow, 1995-1997, New York Medical College

  • Presidential award, 1996, Great Lakes International Imaging and Flow Cytometry Association. Detroit, Michigan.

  • Quantitative Image Cytometry (QIC) Center. Member of the Advisory Board, since April 2007.

  • The 2007-2208 Excellence in Science Award – Scientific Breakthrough – in recognition to distinguished service to the Departments of Medical Sciences and Clinical Immunology, Amgen Inc.

  • 2010 P&PD Cross functional collaboration Award – Immunogenicity of Protein Aggregates”. Amgen Inc.

  • 2016 Translational Sciences Award – “Delivers Results”. Amgen Inc.

ADVISOR/MENTOR

  • Adjunct Professor at the University of New Haven, West Haven, CT (mentor a master thesis)-1999

  • Thesis advisor of two graduate students (MD-PhD program), University of Valencia, Spain- 1995  

AFFILIATIONS

  • International Society for the Advancement of Cytometry (ISAC); Member ID: 1164730

  • American Association for Cancer Research (AACR); Member ID: 289859 

REFEREE OF MANUSCRIPTS

  • Cytometry

  • Cell Proliferation

  • Experimental Cell Research

  • American Journal of Pathology

PATENTS 

  • Separation of nucleic acids by antibodies towards halogenated nucleotides. European Patent Office. Patent No.  98932774.7-2116. WO 99/06592.

  • Physical separation of nucleic acids by antibodies to halogenated nucleotides. Patent No.: US 6417343 (Date of Patent: Jul.9, 2002)

  • Flow cytometric methods for the concurrent detection of discrete functional conformation of pRB in single cells. Patent No.: US 6821740 (Date of Patent: Nov. 23, 2004)

  • Detection of protein conformations in single cells. Patent No.: US 7070943 (Date of Patent: Jul.4, 2006)

PUBLICATIONS

  1. Juan G, Cavazzoni M, Sáez GT and O'Connor JE. A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry. Cytometry 15:335-342 (1994).

  2. Juan G, Gil-Benso R, O’Connor JE and Callaghan RC. Oxidative Metabolism in a Rat Hepatoma (N13) and Isolated Hepatocytes: A Flow Cytometric Comparative Study. Hepatology,24:385-30 (1996).

  3. Sastre J, Pallardó FV, Plá R, Pellin A, Juan G, O'Connor JE, Estrela JM, Miquel J and Viña J. Aging of the liver: age-associated mitochondrial damage in intact hepatocytes. Hepatology, 24:1199-1205 (1996).

  4. Darzynkiewicz Z, Gong J, Juan G, Ardelt B and Traganos F. Cytometry of Cyclin Proteins. Cytometry, 25:1-13 (1996).

  5. Juan G, Gong J, Traganos F and Darzynkiewicz Z. Unscheduled Expression of Cyclins D1 and D3 in Human Tumor Cell Lines. Cell Prolif., 29:259-266 (1996).

  6. Juan G, Pan W and Darzynkiewicz Z. DNA Segments Sensitive to Single Strand Specific Nucleases are Present in Chromatin of Mitotic Cells. Exp. Cell Res.,227:197-202 (1996).

  7. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T and Traganos F. Cytometry in Cell Necrobiology: Analysis of Apoptosis and Accidental Cell Death (Necrosis). Cytometry, 27:1-20 (1997).

  8. Juan G, Li X and Darzynkiewicz Z. Correlation between DNA Replication and Expression of Cyclins A and B1 in Individual MOLT-4 Cells. Cancer Res., 57:803-807 (1997).

  9. Vibhakar R, Juan G, Traganos F, Darzynkiewicz Z and Finger L. Activation-Induced Expression of Human Programmed Death-1 Gene in T lymphocytes. Exp. Cell Res.,232:25-28 (1997).

  10. Halicka HD, Juan G, Mittelman A, Chen S, Traganos F and Darzynkiewicz Z. Apoptosis and Cell Cycle Effects Induced by Extracts of the Chinese Herbal Preparations PC SPES. In. J. Oncol., 11:437-448 (1997).

  11. Gorczyca W, Sarode V, Juan G, Melamed MR and Darzynkiewicz Z. Laser Scanning Cytometric Analysis of cyclin B1 in Primary Human Malignancies. Mod. Pathol. 10:457-462 (1997).

  12. Haider SR, Juan G, Traganos F and Darzynkiewicz Z. Immunoseparation and Immunodetection of nucleic acids labeled with halogenated nucleotides. Exp. Cell Res., 234: 498-506 (1997).

  13. Juan G and Darzynkiewicz Z. Detection of cyclins in individual cells by flow and laser scanning cytometry. In: Methods in Molecular Biology, V XX (22). Flow Cytometry Protocols, Jaroczeski and Heller, eds. Humana Press, Inc. Clifton, NJ, pp: 1-9 (1997).

  14. Juan G and Darzynkiewicz Z. The Detection of Apoptosis and DNA Replication by Differential Labeling of DNA Strand Breaks with Fluorochromes of Different Color. Apoptosis and Programmed Cell Death Protocols. Graham Cowling, Ed. In: Molecular Medicine Series. Humana Press, Inc. Clifton, NJ. (1997).

  15. Sastre J, Millan A, Garcia de la Asuncion J, Pla R, Juan G, Pallardo FV, O'Connor JE, Martin JA, Droy-Lefaix  MT and Vina J. A Ginkgo Biloba Extract (EGb 761) prevents mitochondrial aging by protecting against oxidative stress. Free Rad. Biol. Med., 298-304 (1998).

  16. Juan G, Gruenwald S and Darzynkiewicz Z. Phosphorylation of retinoblastoma susceptibility gene protein assayed in individual lymphocytes during their mitogenic stimulation. Exp. Cell Res., 239: 104-110 (1998).

  17. Juan G, Traganos F, James WM, Ray JM, Roberge M, Sauve DM, Anderson H and Darzynkiewicz Z. Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry, 32: 1-8 (1998).

  18. Juan G, Ardelt B, Mikulski SM, Shogen K, Ardelt W, Mittelman A and Darzynkiewicz Z. G1 arrest of U937 cells by Onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP, and decreased pRb phosphorylation. Leukemia, 12: 1241-1248 (1998).

  19. Juan G, Li X and Darzynkiewicz Z. Phosphorylation of retinoblastoma protein (pRb) assayed in individual HL-60 cells during their proliferation and differentiation. Exp. Cell Res., 244:83-92 (1998).

  20. Juan G and Darzynkiewicz Z. Phosphorylation of retinoblastoma protein (pRb) assayed in individual cells by multilaser flow cytometry. Clin. Immunol. Newslett., 18: 89-94 (1998).

  21. Juan G and Darzynkiewicz Z. In Situ DNA Strand Break Labeling for Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry. Cell Biology. A Laboratory Handbook. 2nd Edition. Julio E. Celis, ed. Academic Press, San Diego, CA. pp: 335-344 (1998).

  22. Juan G and Darzynkiewicz Z. Cell Cycle Analysis by Flow and Laser Scanning Cytometry. Cell Biology. A Laboratory Handbook. 2nd Edition. Julio E. Celis, ed. Academic Press, San Diego, CA. pp: 255-268 (1998).

  23. Darzynkiewicz Z and Juan G. Selective extraction of fragmented DNA from apoptotic cells for analysis by gel electrophoresis and identification of apoptotic cells by flow cytometry. In: Methods Mol. Biol. "DNA Repair Protocols: Eukariotic Systems". DS Henderson, ed. Humana Press. Chapter 50: (1998).

  24. Juan G, Traganos F and Darzynkiewicz Z. Histone H3 phosphorylation in human monocytes and during HL-60 differentiation. Exp. Cell Res., 246:212-220 (1999).

  25. Hsieh T-C, Juan G, Darzynkiewicz Z and Wu JM. Resveratrol increases nitric oxide synthase, induces accumulation of p53 and p21 and suppresses cultured bovine pulmonary artery endothelial cell proliferation by perturbing progression through S and G2. Cancer Res., 59: 2596-2601 (1999).

  26. Juan G, Traganos F. and Darzynkiewicz Z. Cytometric analysis of the pRb pathway. In: The cell cycle: a practical approach. G. Studzinski, ed., Oxford University press, Oxford, UK. pp: 145-159 (1999).

  27. Albino AP, Juan G, Traganos F, Reinhart L, Connolly J, Rose DP and Darzynkiewicz Z. Cell cycle arrest and apoptosis of melanoma cells by decosahexanoic acid: association with decreased pRB phosphorylation. Cancer Res., 60:4139-4145 (2000).

  28. DiPietratonio AM, Hsieh T-C, Juan G, Traganos F, Darzynkiewicz Z and Wu JM. Fenretinide-induced Caspase 3 activity involves increased protein stability in a mechanism distinct from reactive oxigen species elevation. Cancer Res., 60:4331-4335 (2000).

  29. Juan G and Cordon-Cardo C. Intranuclear compartmentalization of cyclin E during the cell cycle: Disruption of the nucleoplasm-nucleolar shuttling of cyclin E in bladder cancer. Cancer Res. 61:1220-1226(2001).

  30. Paris F, Fuks Z, Kang A, Capodieci P, Juan G, Ehleiter D, Haimovitz-Friedman A, Cordon-Cardo C and Kolesnick R. Endothelial apoptosis is the primary lesion initiating radiation damage to the intestines. Science 293:293-297(2001).

  31. Mora J, Cheung N-KV, Juan G, Illei P, Chi S, Akram M, Chen L, Cheung I, Ladanyi M, Cordon-Cardo C and Gerald Wl. Neuroblastoma tumor cell subtypes originate from a stem cell capable of developing along multiple lineages. Cancer Res. 61:6892-6898 (2001).

  32. Juan G, Traganos F and Darzynkiewicz Z. Methods to identify mitotic cells by flow cytometry.  Methods Cell Biol. 63: 343-354 (2001).

  33. Darzynkiewicz Z, Juan G and Bedner E. Determining cell cycle stages by flow cytometry. Curr. Protoc. Cell Bio. Chapter 8:Unit 8.4  (2001).

  34. Darzynkiewicz Z, Juan G and Traganos F. Assaying drug-induced apoptosis. Methods Mol Biol. 95:241-54 (2001).

  35. Traganos F, Juan G and Darzynkiewicz Z. Cell cycle analysis of drug treated cells. Methods Mol Biol. 95:229-40 (2001).

  36. Darzynkiewicz Z and Juan G. Overview of nucleic acid analysis. Curr. Protoc. Cytom. Chapter 7: Unit 7.1  (2001).

  37. Darzynkiewicz Z and Juan G. Analysis of DNA content and DNA strand breaks for detection of apoptotic cells. Curr. Protoc. Cytom. Chapter 7:Unit 7.4  (2001).

  38. Darzynkiewicz Z and Juan G. DNA content measurement for DNA ploidy and cell cycle analysis. Curr. Protoc. Cytom. Chapter 7:Unit 7.5  (2001).

  39. Darzynkiewicz Z and Juan G. Analysis of DNA content and BrdUrd incorporation. Curr. Protoc. Cytom. Chapter 7:Unit 7.7  (2001).

  40. Darzynkiewicz Z and Juan G. Analysis of DNA denaturation. Curr. Protoc. Cytom. Chapter 7:Unit 7.8  (2001).

  41. Juan G and Darzynkiewicz Z. Bivariate analysis of DNA content and expression of cyclin proteins. Curr. Protoc. Cytom. Chapter 7:Unit 7.9 (2001).

  42. Juan G, Hernando E and Cordon-Cardo C. Separation of live cells in different phases of the cell cycle for gene expression analysis. Cytometry 49:170-175 (2002).

  43. Darzynkiewicz Z, Juan G, Traganos F. Cytometry of cell cycle regulatory proteins. Prog. Cell Cycle Res. 5:533-542 (2003).

  44. Darzynkiewicz Z, Juan G and Traganos F. Cytometry of cell cycle regulatory proteins.  In: Progress in Cell Cycle Research, Vol. 5. L. Meijer, A. Jezequel and M .Roberge, Eds). Vol. 5, pp. 533-542 (2003).

  45. Hernando E,Nahle Z, Juan G, Diaz-Rodriguez E, Alaminos M, Hemann M, Michel L, Mittal V, Gerald W, Benezra R, Lowe SW and Cordon-Cardo C. Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature 430:797-802 (2004).

  46. Darzynkiewicz Z, Juan G and Srour EF. Differential Staining of DNA and RNA. Curr. Protoc. Cytom. Chapter 7:Unit 7.3 (2004).

  47. Juan G and Darzynkiewicz Z. Detection of mitotic cells. Curr. Protoc. Cytom. Chapter 7: Unit 7. 24. (2004).

  48. Mora J, Lavarino C, Alaminos M, Cheung N-K, Rios J, de Torres C, Illei P, Juan G and Gerald W. Comprehensive analysis of tumoral DNA content reveals clonal ploidy heterogeneity as a marker with prognostic significance in locoregional neuroblastoma.  Genes Chromosomes Cancer 46:385-396 (2007).

  49. Ardelt B, Juan G, Burfeind P, Salomon T, Wu JM, Hsieh TC, Li X, Sperry R, Pozarowski P, Shogen K, Ardelt W and Darzynkiewicz Z. Onconase, an anti-tumor ribonuclease suppresses intracellular oxidative stress. Int J Oncol 31:663-669 (2007).

  50. Zoog S, Itano A, Trueblood E, Pacheco E, Zhou L, Zhang X, Ferbas J, Ng G and Juan G. Antagonists of CD117 (cKit) signaling inhibit Mast Cell Accumulation in Healing Skin Wounds. Cytometry part A, 75:189-198 (2009).

  51. Tulchin N, Chambon M, Juan G, Dikman S, Strauchen J, Ornstein L, Billack B, Woods NT, Monteiro NA.  BRCA1 protein and nucleolin co-localize in breast carcinoma tissue and cancer cell lines. Am. J. Pathol. 176:1203-1214 (2010).

  52. Kaplan-Lefko PJ, Graves JD, Zoog SJ, Pan Y, Wall J, Branstetter DG, Moriguchi J, Coxon A, Huard JN, Xu R, Peach ML,Juan G,Kaufman S,Chen Q,Bianchi A,Kordich JJ,Ma M,Wong‑Madden S,Foltz IN and Gliniak BC.Conatumumab, a fully human agonist antibody to death receptor 5, induces apoptosis via caspase activation in tumors. Cancer Biol.Ther. 9: 618-631 (2010).

  53. Herbst R, Kurzrock R, Hong D, Valdivieso M, Hsu C-P, Goyal L, Juan G, Hwang Y, Wong S, Hill J, Friberg G, and LoRusso P. A first-in-human study of conatumumab in adult patients with advanced solid tumors. Clin. Cancer Res. 16: 5883-5891 (2010).

  54. Zoog SJ, Ma C, Kaplan-Lefko PJ, Hawkins J, Moriguchi J, Zhou L, Pan Y, Hsu C-P, Friberg G, Herbst R, Hill J, and Juan G. Measurement of Conatumumab-induced apoptotic activity in tumors by fine needle aspirate sampling. Cytometry Part A, 77A:849-860 (2010).

  55. Juan G, Zoog SJ and Ferbas J.  Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies. In: Methods in Cell Biology: Recent Advances in Cytometry.  Vol. 102, pp. 309-320 (2011).

  56. Tatarewicz SM, Juan G, Swanson SJ and Moxness MS.  Epitope characterization of pre-existing and developing antibodies to an aglycosylated monoclonal antibody therapeutic of G1m17,1 allotype J. Immunol. Methods. 382:93-100 (2012).

  57. Jiansong Xie J, Ferbas J and Juan G. Three-dimensional Second Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues. Curr. Protoc. Cytom. Chapter 6: Unit 6. 33 (2012).

  58. Zoog SJ, Trueblood E, Zhou K, Ma CY, Pacheco E, Woody L, Ng GY, Dodson S, Banfield C, Juan G and Parnes JR.  Kinetics of dermal mast cell accumulation in wounds healing by second intention. (Submitted).

  59. Juan G. Epigenetics by Flow! (Commentary) Cytometry Part A, 85A: 8-9 (2014).

  60. Kaliyaperumal A, Pennucci J, Nagatani J, Juan G, Swanson S, and Gupta S. A method to quantitate the neutralizing capacity of anti-therapeutic protein antibodies in serum and their correlation to clinical impact. J. Pharm. Biomed. Anal. 102: 176-183 (2015).

  61. Wang SW, Paweletz K, Chastain M, Loberg R and Juan G. A novel MHC-dextramer assay to identify melanoma antigen-specific CD8+ T cells from solid tumors disaggregates and matched peripheral blood. J. Immunother. Cancer, 3(suppl2): P109 (2015).

  62. Juan G. In-Silico Analysis of Cell Cycle Progression (Commentary). Cytometry Part A, 85A: 741-742 (2014).

  63. Juan G, Bush TL, Ma C, Manoukian R, Chung G, Hawkins JM, Zoog S, Kendall R, Radinsky R, Loberg R, Friberg G, and Payton M. AMG 900, a potent inhibitor of aurora kinases causes pharmacodynamic changes in p-Histone H3 immunoreactivity in human tumor xenografts and proliferating mouse tissues. J. Transl. Med. 12: 307-318 (2014).

  64. Kantarjian HM, Schuster MW, Jain N, Advani A, Jabbour E, Gamelin E, Rasmussen E, Juan G, Anderson A, Chow VF, Friberg G, Vogl FD, Sekeres MA. A Phase 1 study of AMG 900, an orally administered pan-Aurora kinase inhibitor, in adult patients with acute myeloid leukemia. Am. J. Hematol. 92: 660-667(2017).

  65. Paweletz K, Trueblood E, Anderson A, Hensley K, Deshpande C, Rossi J, Damore M, Loberg R, and Juan G. EGFR-BiTE® antibodies connect resident infiltrating T cells with EGFR-expressing cancer cells for redirected target cell lysis in an ex vivo model of human primary tumors. (Manuscript submitted to CII 2017).

  66. Carducci M, Shaheen M, Markman B, Hurvitz S, Mahadevan D, Kotasek D, Goodman OB, Rasmussen E, Chow V, Juan G, Friberg GR, Gamelin E, Vogl FD, Desai J. A Phase 1, First-in-Human study of AMG 900, an orally administered pan-Aurora kinase inhibitor, in adult patients with advanced solid tumors. Invest. New Drugs. 36: 1060-1071 (2018).

  67. Tran B, Richard D. Carvajal RD, Marabelle A, Patel SP, LoRusso PM, Rasmussen E, Juan G, Upreti VV, Beers C, Ngarmchamnanrith G, Scöffski P. Dose escalation results from a first-in-human, phase 1 study of glucocorticoid-induced TNF receptor–related protein agonist AMG 228 in patients with advanced solid tumours. J. Immunother. Cancer. 6: 93 (2018).

  68. Payton M, Cheung K, Ninniri S, Marinaccio C, Wayne W, Hanestad K, Crispino JD, Juan G, Coxon A. Dual targeting of aurora kinases with AMG 900 exhibits potent preclinical activity against acute myeloid leukemia with distinct post-mitotic outcomes. Mol. Cancer Ther. 17: 2575-2585 (2018).

  69. Juan G. Could the key be hidden in the lysosomes? (Commentary). Cytometry Part A. 95: 712-713 (2019).

  70. Razak ARA, Cleary JM, Moreno V, Boyer M, Calvo Aller E, Edenfield W, Tie J, Harvey RD, Rutten A, Shah MA, Olszanski AJ, Jäger D, Lakhani N, Ryan DP, Rasmussen E, Juan G, Wong H, Soman N, Smit M-A D, Nagorsen D, Papadopoulos KP. Safety and efficacy of AMG 820, an anti-colony-stimulating factor 1 receptor antibody, in combination with pembrolizumab in adults with advanced solid tumors. J Immunother Cancer. 8(2): e001006 (2020).

INVITED SPEAKER

  1. First Comett Course on flow cytometry: Analysis of Cell Death by Flow Cytometry. Practical session: "Kinetic approaches to study cell metabolism and toxicity". Université Claude Bernard, Lyon, France, 1993.

  2. Post-graduate courses on Clinical Applications of Flow Cytometry (3 courses). Department of Biochemistry and Molecular Biology. Faculty of Medicine, University of Valencia.

  3. Training courses for EPICS Profile II flow cytometer (25 courses) and EPICS Elite cell sorter (5 courses). Reference Center for Flow Cytometry. Dept. of Biochemistry and Molecular Biology. Faculty of Medicine, University of Valencia (200-2003).

  4. ISAC XVIII Congress, 13-18 April, 1996, Palacongressi, Rimini (Italy). Platform presentation in Theme II Cell Cycle/Apoptosis

  5. Professor at the Annual Course in Flow Cytometry. Applications in Immunology and Cell Biology". Brunswick, ME.  June 9-14, 1996.

  6. Great Lakes International Imaging and Flow Cytometry Association, fifth annual meeting, 4-6 October, 1996. Presidential symposium presentation. "Scheduled and Unscheduled Expression of Cyclins B1, A, E and D in Normal and Tumor Cell Lines”.

  7. Clinical Applications of Cytometry. Charleston, SC, 21-24 August, 1996. "Cytometry of Cyclin Proteins"

  8. Compucyte New Technology Seminar. 16 July, 1996. "Cytometry of Cyclin Proteins"

  9. V Congresso da sociedade iberica de cytometria. 3 June, 1997. "Apoptosis versus proliferation".

  10. Sam Latt Conference (ISAC). Toronto (Canada) 20-22 June. "Correlation between DNA replication of cyclins E, A, B1 as well as PCNA and Ki-67 in individual MOLT-4 cells".

  11. Professor at the flow cytometry workshop on the simultaneous analysis of the cell cycle and cyclin expression. San Juan, PR. April 25-29, 1997.

  12. ISAC XIX Congress, 28 February-5 March, 1998, The Broadmoor Hotel in Colorado Springs, Colorado. Platform presentation in Theme II Cell Cycle.  

  13. ISAC XIX Congress, 28 February-5 March, 1998, The Broadmoor Hotel in Colorado Springs, Colorado. Platform presentation in Theme II Cell Cycle. 

  14. 51st annual meeting of the Histochemical Society. New Orleans, LA, March 24-25, 2000. Title: Visualizing the cell cycle in human malignant tumors.

  15. QIC Cold Spring Harbor Symposium. Title: An automated method to monitor skin mast cells by laser scanning cytometry. CSHL, NY. February 21st, 2007.

  16. QIC Cold Spring Harbor Symposium. Title: Quantitation of Caspase3 Activation as a Pharmacodynamic Endpoint in Fine Needle Aspirate (FNA) Biopsies. CSHL, NY. February 1st, 2008.

  17. NW Regional Cytometry meeting. Title: Coupling Fine Needle Aspirates with Imaging Cytometry to Sample Caspase-3 Activation in Tumors. Fred Hutchinson Cancer Research Center. Seattle, WA. March 14, 2009.

  18. QIC Children’s Hospital Boston. Title: Leveraging Quantitative Imaging Cytometry to Measure the PD Impact of Drugs in Development: Antagonists of CD117 signaling inhibit mast cell accumulation in healing skin wounds. Boston, MA. August 12th, 2010.

  19. CYTO2011. Title: Imaging Cytometry in Biomarker Drug Development. Baltimore, MD. May 21st, 2011.